Supplemental material

Promoter associated RNAs

Stefanie Wehner, Anja K. Dörrich, Philipp Ciba, Annegret Wilde, and Manja Marz

Overview

Data from literature
Databases used for this work
Gff and Fasta files
Alignment
Overview of pRNA locus
Conserved boxes
Detailed protocol of Cell isolation, Cell culture and RNA isolation
Additional experimental analyses
Designed primers

Data from literature

sequences.fa
sequences.aln

Databases used for this work

f-chromosom; s-scaffold/superscaffold; c-contig/supercontig
Organism AssemblyCoverage real genomesize (Mbp) Mbp expected Download date Download
Homo sapiens f 3168 3423 3.06.12 Download link
Pan troglodytes f 6X 3310 3594 5.06.12 Download link
Pongo pygmaeus f 6X 3447 3703 3.07.12 Download link
Macaca mulatta f 5.1X 3097 3291 7.06.12 Download link
Callithrix jacchus Marmoset c 6X 2915 3355 15.06.12 Download link
Tarsius syrichta s 1.82X 3187 5144 4.07.12 Download link
Otolemur garnettii s 2519 3531 7.06.12 Download link
Microcebus murinus s 1.93X 2910 3051 4.07.12 Download link
Mus musculus f 25X 2655 3216 20.06.12 Download link
Rattus norvegicus f 2719 3286 7.06.12 Download link
Dipodomys ordii s 1.85X 2165 4377 4.07.12 Download link
Spermophilus tridecemlineatus s 2478 3932 4.07.12 Download link
Cavia porcellus s 6.79X 2723 3751 4.07.12 Download link
Ochotona princeps s 1.93X 4782 3107 4.07.12 Download link
Oryctolagus cuniculus s 7.48 2737 3107 1.06.12 Download link
Tupaia belangeri s 2X 3670 3472 7.06.12 Download link
Felis catus s 2.87X 3160 3040 4.07.12 Download link
Canis familiaris f 7.6X 2532 3051 26.06.12 Download link
Vicugna pacos s 2.51X 2968 3169 26.06.12 Download link
Tursiops truncatus s 2.59X 2522 3081 1.06.12 Download link
Bos taurus f 9.5X 2670 3537 4.07.12 Download link
Sus scrofa f 15.3X 2809 3059 5.06.12 Download link
Equus caballus f 6.8X 2475 3149 25.05.12 Download link
Myotis lucifugus s 7X 2035 2248 29.05.12 Download link
Pteropus vampyrus s 2.63X 2000 2318 20.06.12 Download link
Erinaceus europaeus s 1.86X 3368 3579 3.07.12 Download link
Sorex araneus s 1.9X 2944 2846 3.07.12 Download link
Echinops telfairi s 2X 3824 4919 15.06.12 Download link "
Procavia capensis s 2.19X 2993 3971 4.07.12 Download link
Dasypus novemcinctus s 2X 4824 5291 3.07.12 Download link
Choloepus hoffmanni s 2.05X 2467 4137 29.05.12 Download link
Monodelphis domestica f 7.33X 3606 4592 20.6.12 Download link
Macropus eugenii s 2X 2956 4224 15.6.12 Download link
Ornithorhynchus anatinus f 6 2073 2993 26.6.12 Download link
Anolis carolinensis T f 7.1X 1799 2242 22.5.12 Download link
Taeniopygia guttata f 6X 1233 1222 6.8.12 Download link
Gallus gallus f 7.1X 1108 1222 8.6.12 Download link


Gff and Fasta files

pRNA rRNA
Organism gff fasta gff
Homo sapienspRNA.gff (93) pRNA.fa rRNA.gff (2)
Pan troglodytespRNA.gff (95) pRNA.fa rRNA.gff (15)
Pongo pygmaeuspRNA.gff (84) pRNA.fa rRNA.gff (1)
Macaca mulatapRNA.gff (89) pRNA.fa rRNA.gff (2)
Callithrix jacchus MarmosetpRNA.gff (60) pRNA.fa rRNA.gff (2)
Tarsius syrichtapRNA.gff (58) pRNA.fa
Otolemur garnettiipRNA.gff (30) pRNA.fa
Microcebus murinuspRNA.gff (102) pRNA.fa rRNA.gff (1)
Mus musculuspRNA.gff (8) pRNA.fa rRNA.gff (1)
Rattus norvegicuspRNA.gff (5) pRNA.fa
Dipodomys ordiipRNA.gff (45) pRNA.fa rRNA.gff (5)
Spermophilus tridecemlineatuspRNA.gff (23) pRNA.fa
Cavia porcelluspRNA.gff (11) pRNA.fa rRNA.gff (2)
Ochotona princepspRNA.gff (53) pRNA.fa rRNA.gff (7)
Oryctolagus cuniculuspRNA.gff (21) pRNA.fa rRNA.gff (4)
Tupaia belangeripRNA.gff (46) pRNA.fa rRNA.gff (6)
Felis catuspRNA.gff (60) pRNA.fa rRNA.gff (1)
Canis familiarispRNA.gff (53) pRNA.fa rRNA.gff (3)
Vicugna pacospRNA.gff (55) pRNA.fa rRNA.gff (1)
Tursiops truncatuspRNA.gff (102) pRNA.fa rRNA.gff (1)
Bos tauruspRNA.gff (46) pRNA.fa
Sus scrofapRNA.gff (51) pRNA.fa
Equus caballuspRNA.gff (130) pRNA.fa rRNA.gff (5)
Myotis lucifuguspRNA.gff (50) pRNA.fa rRNA.gff (2)
Pteropus vampyruspRNA.gff (67) pRNA.fa rRNA.gff (4)
Erinaceus europaeuspRNA.gff (23) pRNA.fa rRNA.gff (2)
Sorex araneuspRNA.gff (17) pRNA.fa rRNA.gff (1)
Echinops telfairipRNA.gff (23) pRNA.fa rRNA.gff (1)
Procavia capensispRNA.gff (297) pRNA.fa rRNA.gff (2)
Dasypus novemcinctuspRNA.gff (53) pRNA.fa rRNA.gff (5)
Choloepus hoffmannipRNA.gff (69) pRNA.fa


Alignment

pRNA_with_boxes.stk
pRNA_with_boxes.pdf

Overview of pRNA locus

transcript

Conserved boxes

A-Box

A-Box

B-Box

B-Box

Detailed protocol of Cell isolation, Cell culture and RNA isolation

protocoll.pdf

Additional experimental analyses

Additional analyses

Designed primers

Primers for the pRNAs
Primers for the boxes