List of Projects

    Bioinformatics support for researchers of the FSU and associated research institutes

    The Bioinformatics Core Facility Jena (BiC) provides free support for researchers of the Friedrich Schiller University and associated research institutes in Jena at all stages of bioinformatics analysis. The support we offer ranges from consultations, basic bioinformatics services, and scientific workshops to full research collaborations. For our basic bioinformatics services, we have established modern, standardized workflows for numerous tasks in the field of high-throughput analysis and related research areas, starting with data quality control up to the final visualization of the results. For special applications, where our standardized methods reach their limits or a deeper interpretation of the results is desired, we offer individually adapted solutions in the form of full research collaboration. In the end, our aim is to contribute to the interdisciplinarity and development of life science research projects in Jena. Through our still-growing network of scientific partners, we have the opportunity to work on a huge variety of different topics.

    People involved: Emanuel Barth, Muriel Ritsch, Sebastian Krautwurst, Daria Meyer

    Viruses in groundwater

    The principle aim of the CRC AquaDiva is to increase our understanding of the links between surface and subsurface, especially how organisms inhabiting the subsurface critical zone reflect and influence their physical, ecological and geochemical environment. In project A06, we will determine the diversity of previously known viruses in groundwater by high-throughput sequencing of viral genomes. A challenge in virology is the identification of previously undetected viruses, which we will tackle with our new approach to virus assembly. We complement the approach by investigating the differences in metatranscriptomes of the different sampling sites. Finally, we will determine the broadly unknown virus decay in groundwater to gain insights into communication pauses with other organisms.

    People involved: Christian Höner zu Siederdissen, Akash Srivastava, Sebastian Krautwurst, Muriel Ritsch, Milena Žarković

    Collaborations: Kirsten Küsel, Antonis Chatzinotas, René Kallies, Christian Jogler

    Grants: CRC 1076 — AquaDiva: A6: Viral Diversity, Viral de novo Assembly, and Viral Decay in Groundwater

    Related publications:

    Overholt, Will A.; Trumbore, Susan; Xu, Xiaomei; Bornemann, Till L. V.; Probst, Alexander J.; Krüger, Markus; Herrmann, Martina; Thamdrup, Bo; Bristow, Laura A.; Taubert, Martin; Schwab, Valérie F.; Hölzer, Martin; Marz, Manja; Küsel, Kirsten

    Carbon fixation rates in groundwater similar to those in oligotrophic marine systems Journal Article

    In: Nat Geosci, vol. 15, pp. 561–567, 2022.

    Abstract | Links | BibTeX

    Chaudhari, Narendrakumar M.; Overholt, Will A.; Figueroa-Gonzalez, Perla Abigail; Taubert, Martin; Bornemann, Till L. V.; Probst, Alexander J.; Hölzer, Martin; Marz, Manja; Küsel, Kirsten

    The economical lifestyle of CPR bacteria in groundwater allows little preference for environmental drivers Journal Article

    In: Environ Microbiome, vol. 16, no. 1, pp. 24, 2021.

    Abstract | Links | BibTeX

    Overholt, Will A.; Hölzer, Martin; Geesink, Patricia; Diezel, Celia; Marz, Manja; Küsel, Kirsten

    Inclusion of Oxford Nanopore long reads improves all microbial and viral metagenome-assembled genomes from a complex aquifer system Journal Article

    In: Environ Microbiol, vol. 22, no. 9, pp. 4000-4013, 2020.

    Abstract | Links | BibTeX

    Kallies, René; Hölzer, Martin; Toscan, Rodolfo Brizola; da Rocha, Ulisses Nunes; Anders, John; Marz, Manja; Chatzinotas, Antonis

    Evaluation of Sequencing Library Preparation Protocols for Viral Metagenomic Analysis from Pristine Aquifer Groundwaters. Journal Article

    In: Viruses, vol. 11, no. 6, pp. 484, 2019.

    Abstract | Links | BibTeX

    Wegner, Carl-Eric; Gaspar, Michael; Geesink, Patricia; Herrmann, Martina; Marz, Manja; Küsel, Kirsten

    Biogeochemical regimes in shallow aquifers reflect the metabolic coupling of elements of nitrogen, sulfur and carbon. Journal Article

    In: Appl Environ Microbiol, vol. 85, no. 5, pp. e02346-18, 2019.

    Abstract | Links | BibTeX

    Starke, Robert; Müller, Martina; Gaspar, Michael; Marz, Manja; Küsel, Kirsten; Totsche, Kai Uwe; Bergen, Martin; Jehmlich, Nico

    Candidate Brocadiales dominates C, N and S cycling in anoxic groundwater of a pristine limestone-fracture aquifer Journal Article

    In: J Proteomics, vol. 152, pp. 153–160, 2016.

    Abstract | Links | BibTeX

    Ecology and species barriers in emerging viral diseases

    Emerging viruses existing in animal reservoirs may cause epidemic or epizootic diseases if transmitted to humans or livestock. While we understand the pathogenicity and epidemiology of prototypic emerging viral diseases, we know little about the mechanisms driving virus emergence from animal reservoirs. To move ahead, we need to generalize our view on emerging viruses, taking into consideration the ecology of viruses in their natural reservoirs. We hypothesize that small mammals, mainly bats, and rodents, constitute the most relevant virus reservoirs due to their large group sizes, population density, mixing, and turnover, as well as their exposure to arthropod vectors.

    People involved: Marie Lataretu, Gabriel Lencioni Lovate, Daria Meyer

    Past people involved: Martin Hölzer, Nelly Fernanda Mostajo Berrospi

    Collaborations: Christian Drosten, Friedemann Weber, Stephan Becker, Martin Beer, Georg Kochs

    Grants: DFG SPP 1596: Ecology and Species Barriers in Emerging Viral Diseases

    Related publications:

    Mostajo, Nelly F.; Lataretu, Marie; Krautwurst, Sebastian; Mock, Florian; Desirò, Daniel; Lamkiewicz, Kevin; Collatz, Maximilian; Schoen, Andreas; Weber, Friedemann; Marz, Manja; Hölzer, Martin

    A comprehensive annotation and differential expression analysis of short and long non-coding RNAs in 16 bat genomes Journal Article

    In: NAR Genomics Bioinf, vol. 2, no. 1, pp. lqz006, 2019.

    Abstract | Links | BibTeX

    Hölzer, Martin; Krähling, Verena; Amman, Fabian; Barth, Emanuel; Bernhart, Stephan H.; Carmelo, Victor A. O.; Collatz, Maximilian; Doose, Gero; Eggenhofer, Florian; Ewald, Jan; Fallmann, Jörg; Feldhahn, Lasse M.; Fricke, Markus; Gebauer, Juliane; Gruber, Andreas J.; Hufsky, Franziska; Indrischek, Henrike; Kanton, Sabina; Linde, Jörg; Mostajo, Nelly F.; Ochsenreiter, Roman; Riege, Konstantin; Rivarola-Duarte, Lorena; Sahyoun, Abdullah H.; Saunders, Sita J.; Seemann, Stefan E.; Tanzer, Andrea; Vogel, Bertram; Wehner, Stefanie; Wolfinger, Michael T.; Backofen, Rolf; Gorodkin, Jan; Grosse, Ivo; Hofacker, Ivo; Hoffmann, Steve; Kaleta, Christoph; Stadler, Peter F.; Becker, Stephan; Marz, Manja

    Differential transcriptional responses to Ebola and Marburg virus infection in bat and human cells Journal Article

    In: Sci Rep, vol. 6, pp. 34589, 2016.

    Abstract | Links | BibTeX

    Limiting viral outbreaks with Machine Learning

    Zoonosis, the natural transmission of infections from animal to human, is a far-reaching global problem, occurring more often due to globalization. In case of a virus outbreak, it is helpful to know which host organism was the original carrier of the virus, so people can be separated from these hosts. The sooner this happens, the greater the chances of limiting an outbreak.
    As a fast method of predicting the original host, we are working on the ability to predict the host of a virus based on the viral genome sequence. Due to the lack of knowledge about virus adaptation, it is difficult to find practical features for machine learning methods. With this in mind, we apply deep learning methods because they do not require predefined features and are one of today’s most powerful machine learning methods.

    People involved: Florian Mock

    Past people involved: Adrian Viehweger

    Related publications:

    Mock, Florian; Viehweger, Adrian; Barth, Emanuel; Marz, Manja

    VIDHOP, viral host prediction with Deep Learning Journal Article

    In: Bioinformatics, vol. 37, no. 3, pp. 318–325, 2020.

    Abstract | Links | BibTeX

    Viruses in host genomes

    Overall, little is known about the composition of the human genome. For example, it is assumed that 50% consist of repetitive sequences, whose function is still undescribed. These include SINEs or LINEs, which are thought to be of viral origin. Another component of the genome is the rarely described Endogenous Viral Elements (EVEs). These can be divided into rarely analyzed retrotransposons and other previously undescribed viral elements. Normally, retroviruses infect somatic cells and integrate their genetic material into the host genome. Infrequently, retroviruses are integrated into germline cells, in which case the viral genome can be passed on to offspring by sexual transmission. This process of integration is called endogenization. It was assumed that only retroviral viruses could integrate into the host genome (since for these viruses, integration was a necessary step in their life cycle). Surprisingly, several non-retroviral elements have already been found in different genomes, but these are confirmed individual occurrences, and there is no detailed catalog of EVEs. Little is known about the integration of non-retroviral EVEs. It has been frequently reported that the human genome harbors 8% viral elements. However, this number refers only to retroviruses and is almost 20 years old. At that time, much less was known about viruses and their replication mechanisms. Sequencing techniques and bioinformatic programs have also evolved enormously since then. It is therefore very surprising that, despite the description of individual non-retroviral elements in the human genome, there has been no fundamental systematic revalidation of the viral elements in the human genome that has occurred.

    In their project, we address three fundamental questions: (1) How many viral elements are there in the human genome? (2) Are they functional? (3) Are the viral fragments accumulated over a human lifetime, or are they essentially inherited?

    People involved: Muriel Ritsch

    RNA structures and functions in viruses

    Non-coding RNAs (ncRNA) are known regulatory elements in organisms from all kingdoms. The secondary structure of RNA is often linked to its function. When looking at a viral genome (especially an RNA genome) it only makes sense that viruses make strong use of ncRNAs to bypass the host’s immune response, regulate their own genes, or stop the gene expression of the host genes. We are concerned with the analysis of conserved RNA structures in well-described virus families and the de novo prediction of potentially functional structural elements in less known families. We use combinations of machine learning, clustering, and homology-based methods. The identification of functional structural elements could help to develop new antiviral therapies in the future, as important replication mechanisms of the virus can be disturbed.

    People involved: Kevin Lamkiewicz, Gabriel Lovate, Sandra Triebel

    Related publications: 

    Triebel, Sandra; Lamkiewicz, Kevin; Ontiveros, Nancy; Sweeney, Blake; Stadler, Peter F.; Petrov, Anton I.; Niepmann, Michael; Marz, Manja

    Comprehensive survey of conserved RNA secondary structures in full-genome alignment of Hepatitis C virus Journal Article

    In: Scientific Reports, vol. 14, iss. 1, 2024.

    Abstract | Links | BibTeX

    Krautwurst, Sarah; Lamkiewicz, Kevin

    RNA-Protein Interaction Prediction without High-Throughput Data: An Overview and Benchmark of in silico Tools Journal Article

    In: bioRxiv, 2024.

    Abstract | Links | BibTeX

    Triebel, Sandra; Lamkiewicz, Kevin; Ontiveros, Nancy; Sweeney, Blake; Stadler, Peter F.; Petrov, Anton I.; Niepmann, Michael; Marz, Manja

    Comprehensive Survey of Conserved RNA Secondary Structures in Full-Genome Alignment of Hepatitis C Virus Journal Article

    In: bioRxiv, 2023.

    Abstract | Links | BibTeX

    Lamkiewicz, Kevin

    RNA secondary structures in RNA viruses: Why viruses would not exist without RNA secondary structures PhD Thesis


    Links | BibTeX

    Jordan-Paiz, Ana; Nevot, Maria; Lamkiewicz, Kevin; Lataretu, Marie; Franco, Sandra; Marz, Manja; Martinez, Miguel Angel

    HIV-1 lethality and loss of Env protein expression induced by single synonymous substitutions in the virus genome intronic splicing silencer Journal Article

    In: J Virol, vol. 94, no. 21, 2020.

    Abstract | Links | BibTeX

    Madhugiri, Ramakanth; Karl, Nadja; Petersen, Daniel; Lamkiewicz, Kevin; Fricke, Markus; Wend, Ulrike; Scheuer, Robina; Marz, Manja; Ziebuhr, John

    Structural and functional conservation of cis-acting RNA elements in coronavirus 5'-terminal genome regions Journal Article

    In: Virology, vol. 517, pp. 44–55, 2017.

    Abstract | Links | BibTeX

    Deciphering the RNA genome packaging code of influenza A viruses

    Currently, bioinformatical tools are not specifically designed for viruses. However, viruses bring unique features, which require specific bioinformatical tools to trace virus-host interaction. For example, the number of sequences in quasispecies is massively high due to their high mutation rate, but only a few interact again with the host cells. Some viruses, such as IAV or as used by AG, are segmented RNA viruses, which urgently require tools with specific features: RNA viruses should include standardized secondary structure predictions, leading to RNA-RNA interaction prediction necessary for the packaging of segmented RNA viruses.

    The aims of this project are to develop a bioinformatical tool to predict RNA-RNA interactions as packaging signal for segmented viruses, such as IAV, to develop a virus-specific full genome multiple sequence alignment algorithm to track the quasispecies; and to establish RNA-RNA interaction sets and more importantly non-interaction sets.

    People involved: Gabriel Lovate, Daniel Desiro, Kevin Lamkiewicz

    Collaborators: Roland Marquet, Andreas Henke

    Grants: HORIZON 2020 MSCA ITN — VIROINF: Understanding (harmful) virus-host interactions by linking virology and bioinformatics. 

    Jakob, Celia; Lovate, Gabriel Lencioni; Desirò, Daniel; Gießler, Lara; Smyth, Redmond Patrick; Marquet, Roland; Lamkiewicz, Kevin; Marz, Manja; Schwemmle, Martin; Bolte, Hardin

    Sequential disruption of SPLASH-identified vRNA–vRNA interactions challenges their role in influenza A virus genome packaging Journal Article

    In: Nucleic Acids Research, 2023, ISBN: 0305-1048.

    Abstract | Links | BibTeX

    Desiro, Daniel

    The complexity of packaging mechanisms in segmented RNA viruses PhD Thesis


    Links | BibTeX

    Desiro, Daniel; Hölzer, Martin; Ibrahim, Bashar; Marz, Manja

    SilentMutations (SIM): a tool for analyzing long-range RNA-RNA interactions in viral genomes and structured RNAs Journal Article

    In: Virus Res, vol. 260, pp. 135-141, 2018.

    Abstract | Links | BibTeX

    Virus Database, interface, and quality control

    The NFDI4Microbiota consortium comprises 10 German partner institutions (including FSU Jena) and aims to build a centralized infrastructure with services for microbiome research. Viruses are a fundamental part of the microbiome and their investigation requires specialized tools and resources. Here at FSU Jena, we are building a virus genome sequence database encompassing all viruses, which will be used by virologists, viral ecologists, and others worldwide in accordance with the FAIR principles. We will do this by consulting the global network of virus experts, integrating expert knowledge and existing database structures, and incorporating international metadata standards. We plan to curate and provide an interface to access the virus genome sequences from public repositories e.g. the European Nucleotide Archive (ENA), the Sequence Read Archive (SRA), and GenBank/NCBI viruses. Further, we will offer visualization, analysis, and sharing of user-uploaded virus data, ensuring data protection for embargoed and private datasets.

    People involved: Shahram Saghaei, Noriko Cassman, Sandra Triebel

    Collaborations: NFDI4Microbiota

    High-Quality Alignments of Viruses

    Multiple sequence alignments (MSAs) reveal homologous regions of input sequences and thus serve as a starting point for phylogenetic analyses at the molecular level. High-quality MSAs can be used to assess the conservation of primary sequence and even secondary structure. In particular, alignments of viral sequences are challenging due to their high mutation rate. Our goal is to create high-quality alignments of viral families and clades by first selecting representative genomes of a dataset by clustering. Then, the alignment is created using current homology-based methods. The final alignment is then used to predict conserved RNA secondary structures using an ILP approach. High-quality alignments of viruses can give insights into differences and similarities at the DNA/RNA level within a virus family. At the same time, providing such information as results of codon corrections (RNA sequence to protein) or compensatory mutations (RNA sequence to RNA secondary structure) is a major difficulty.

    People involved: Sandra Triebel, Kevin Lamkiewicz, Noriko Cassman

    RNA-Protein interactions

    People involved: Sarah Krautwurst, Kevin Lamkiewicz

    Improvement of ONT basecaller

    People involved: Christian Höner zu Siederdissen, Jannes Spangenberg, Sebastian Krautwurst

    Detecting RNA modifications with nanopore sequencing

    RNA modifications such as the highly abundant N6-methyladenosine (m6A) are known as an important aspect of RNA biology. For example, m6A modification has been shown to be involved in the regulation of mRNA processing, but also RNA virus replication and translation. Second-generation sequencing methods for m6A detection are limited to position-only inference on known reference sequences. Nanopore direct RNA sequencing enables the assessment of modification status of individual reads at single-nucleotide resolution, but current detection models are still limited to position-only inference. We aim to use deep neural networks for de-novo modification detection on nanopore data that achieves high accuracy at single read, single-nucleotide resolution.

    People involved: Christian Höner zu Siederdissen, Sebastian Krautwurst, Jannes Spangenberg

    Related publications:

    Meyer, Daria; Goettsch, Winfried; Spangenberg, Jannes; Bohn, Patrick; Stieber, Bettina; Krautwurst, Sebastian; zu Siederdissen, Christian Höner; Srivastava, Akash; Zarkovic, Milena; Wollny, Damian; Marz, Manja

    Maximizing the potential of genomic and transcriptomic studies by nanopore sequencing Journal Article

    In: bioRxiv, 2023.

    Abstract | Links | BibTeX

    Spangenberg, Jannes; zu Siederdissen, Christian Höner; Žarković, Milena; Triebel, Sandra; Rose, Ruben; Christophersen, Christina Martínez; Paltzow, Lea; Hegab, Mohsen M.; Wansorra, Anna; Srivastava, Akash; Krumbholz, Andi; Marz, Manja

    Magnipore: Prediction of differential single nucleotide changes in the Oxford Nanopore Technologies sequencing signal of SARS-CoV-2 samples Journal Article

    In: bioRxiv, 2023.

    Abstract | Links | BibTeX

    Krautwurst, Sebastian; Dijkman, Ronald; Thiel, Volker; Krumbholz, Andi; Marz, Manja

    Direct RNA Sequencing for Complete Viral Genomes Book Section

    In: Frishman, Dmitrij; Marz, Manja (Ed.): Virus Bioinformatics, CRC Press, 2021.

    Abstract | Links | BibTeX

    Viehweger, Adrian; Krautwurst, Sebastian; Lamkiewicz, Kevin; Madhugiri, Ramakanth; Ziebuhr, John; Hölzer, Martin; Marz, Manja

    Direct RNA nanopore sequencing of full-length coronavirus genomes provides novel insights into structural variants and enables modification analysis. Journal Article

    In: Genome Res, vol. 29, pp. 1545-1554, 2019.

    Abstract | Links | BibTeX

    Lechner, Marcus; Marz, Manja; Ihling, Christian; Sinz, Andrea; Stadler, Peter F; Krauss, Veiko

    The correlation of genome size and DNA methylation rate in metazoans Journal Article

    In: Theory Biosci, vol. 132, pp. 47–60, 2012.

    Abstract | Links | BibTeX

    Identifying DNA methylation biomarkers using nanopore sequencing

    Carcinogenesis is associated with DNA methylation changes. Especially the methylation of 5-methylcytosine (5mC) in the context of regions with numerous 5’-cytosine-phosphate-guanine-3’ (CpG) occurrences, so-called CpG islands, plays a role here. These DNA methylation changes occur already at an early stage of cancer. Additionally, it is relatively simple to develop molecular biological tests once the regions of differential methylation are known. Therefore it is convincing to use DNA methylations for cancer screening. Nanopore sequencing makes it possible to identify DNA base modifications (e.g., 5mC) at nucleotide resolution. We aim to develop a workflow to identify DNA methylation cancer biomarkers for cancer subtypes that are not well studied yet.

    People involved: Daria Meyer, Milena Žarković

    Collaborations: oncgnostics GmbH

    Cell-free RNA sequencing

    Cell-free RNA is present in the blood of every human being as a result of vesicular secretion from the cells of the human body. Sequencing cell-free RNA has been very promising for the diagnosis of several diseases ranging from cancer to cardiovascular diseases. In contrast to protein-based biomarkers, a huge advantage of RNA is that it can be amplified. In recent years technological developments enabled the amplification and sequencing of tiny amounts of RNA even from single cells. Another advantage of RNA over DNA (which could also be amplified) is that it is continuously shed from cells. In contrast, DNA exits cells only when the cell is dying. Hence, cell-free RNA holds the promise of continuous probing of transcriptional changes in the cells of the human body. We aim to apply methods from the field of single-cell RNA sequencing to sequence cell-free RNA in different medically relevant contexts. This approach enables the sequencing of minute amounts of RNA and our expertise in sequencing very small amounts of RNA from cells or even phase-separated condensates will facilitate the detection of even the smallest amounts of RNA from the blood or other body fluids.

    People involved: Damian Wollny, Julia Micheel, Aram Safrastyan, Franziska Aron

    Antibiotic resistance in the Ganges river valley

    Antibiotics increasingly fail to treat a growing number of medical conditions due to antimicrobial resistance. This trend is especially acute in developing countries such as India, where broad resistances are known to have emerged. It is known that densely populated cities can drive the emergence and spread of antimicrobial resistance through for example industrial production sites, wastewater management practices, and other cultural characteristics. Proximity to waterways or associated water collections seems especially relevant.
    To identify controllable drivers of resistance emergence and spread we investigate two cities on the river Ganges in India – Allahabad and Kanpur. We also investigate the effect of human interference by analyzing samples before and after Kumbh Mela, which is by far the largest religious gathering in Prayagraj. This will allow us to discern naturally occurring resistance from resistance created by humans.

    People involved: Akash Srivastava, Sebastian Krautwurst

    Collaborations: Ziauddin Ahammad Shaikh, Adrian Viehweger

    Grants: BMBF – DBT Cooperative Science Program: Development of metagenomics assisted surveillance tools for tracking antibiotic resistance in river bodies — A study in the Ganges river valley (NANOLOG)

    Epigenetic profiling of aging mouse brain at base resolution

    Recent studies have proven that epigenetics, especially 5-methylcytosine (5mC), plays a pivotal role in aging. Along these lines, previous studies have reported diverse epigenetic profiles among different cell types like neurons and oligodendrocytes of the same individual. Besides methylation, DNA undergoes various other types of epigenetic modification. It remains to be investigated if these modifications changes upon aging and can thus also serve as an alternative reliable molecular marker of the epigenetic age of an individual. Thus, it is essential to identify variations in other epigenetic modifications of DNA in specific cell types from the same individual. We plan to study various modifications in a single chain reaction using long-read sequencing on the MinIon platform from ONT.

    People involved: Akash Srivastava

    The role of non-coding RNAs in human placental development

    Inside the placenta, the fetal syncytiotrophoblast forms the interface between fetus and mother, from which exosomes and microvesicles are permanently released into the maternal circulation. These particles contain fetal proteins and ncRNAs for communication with neighboring and distant maternal cells. The number, size, and content of these particles may reflect or predict placental disorders. Several severe pregnancy pathologies, including preeclampsia, are human-specific and their pathomechanisms are not yet understood.
    To date, most examples of ncRNAs that have been identified to be specific for fetal tissues, such as the placenta, are members of the group of microRNAs (miRNAs). Long ncRNAs have only been marginally investigated. We need to expand the knowledge about ncRNAs in the placenta and ncRNAs released from it to revolutionize the understanding of regulation processes inside the placenta and of fetal-maternal communication.

    People involved: Sebastian Krautwurst, Milena Žarković, Franziska Hufsky

    Collaborations: Udo Markert, Diana Maria Morales Prieto

    Grants: DFG MA 5082/9-1: Embryonale nicht-kodierende RNAs in der menschlichen Plazenta und dem mütterlichen Blutkreislauf

    Related publications: 

    Murrieta-Coxca, José M; Barth, Emanuel; Fuentes-Zacarias, Paulina; Gutiérrez-Samudio, Ruby N; Groten, Tanja; Gellhaus, Alexandra; Köninger, Angela; Marz, Manja; Markert, Udo R; Morales-Prieto, Diana M

    Identification of altered miRNAs and their targets in placenta accreta Journal Article

    In: Front Endocrinol, vol. 14, pp. 1021640, 2023.

    Abstract | Links | BibTeX

    Žarković, Milena; Hufsky, Franziska; Markert, Udo R; Marz, Manja

    The Role of Non-Coding RNAs in the Human Placenta Journal Article

    In: Cells, vol. 11, iss. 9, pp. 1588, 2022.

    Abstract | Links | BibTeX

    Morales-Prieto, Diana M.; Barth, Emanuel; Murrieta-Coxca, Jose Martín; Favaro, Rodolfo R.; Gutiérrez-Samudio, Ruby N.; Chaiwangyen, Wittaya; Ospina-Prieto, Stephanie; Gruhn, Bernd; Schleußner, Ekkehard; Marz, Manja; Markert, Udo R.

    Identification of miRNAs and associated pathways regulated by Leukemia Inhibitory Factor in trophoblastic cell lines. Journal Article

    In: Placenta, vol. 88, pp. 20–27, 2019.

    Abstract | Links | BibTeX

    Dukhovny, Anna; Lamkiewicz, Kevin; Chen, Qian; Fricke, Markus; Jabrane-Ferrat, Nabila; Marz, Manja; Jung, Jae U.; Sklan, Ella H.

    A CRISPR activation screen identifies genes protecting from Zika virus infection Journal Article

    In: J Virol, vol. 93, no. 16, 2019.

    Abstract | Links | BibTeX

    Morales-Prieto, Diana M.; Barth, Emanuel; Gutièrrez-Samudio, Ruby N.; Chaiwangyen, Wittaya; Ospina-Prieto, Stephanie; Gruhn, Bernd; Schleußner, Ekkehard; Marz, Manja; Markert, Udo R.

    Identification of miRNAs and associated pathways regulated by Leukemia Inhibitory Factor in trophoblastic cell lines Journal Article

    In: bioRxiv, pp. 410381, 2018, (Now published in Placenta:

    Abstract | Links | BibTeX

    Chaiwangyen, Wittaya; Gutiérrez-Samudio, Ruby N.; Markert, Udo R.; Marz, Manja; Morales-Prieto, Diana M.; Ospina-Prieto, Stephanie

    KL 5 Trophoblast-immune cell communication via microRNA transported in extracellular vesicles Journal Article

    In: Pregnancy Hypertens, vol. 9, pp. 5, 2017.


    Statistical modeling of genomic and transcriptomic data

    In the last two decades in the biotechnological area, one revolutionary advancement was chased by another, leading the life sciences into the big data era. However, besides the availability of vast amounts of different biological data, we still lack sufficient statistical models and methods to accurately process and evaluate these data. We aim to develop specialized statistical tools in the context of genomics (e.g., fuzzy k-meres) and transcriptomics (e.g., accurate modeling of read count distributions). We work on different aspects of statistical analysis, starting from the theoretical problem formulation, to the implementation of statistical models and the appropriate visualization of results.

    People involved: Emanuel Barth

    Past Projects